น้ำส้มสายชูเป็นส่วนประกอบสำคัญของน้ำเกลือดอง โดยกรดอะซิติกยังทำหน้าที่เป็นสารกันบูดตามธรรมชาติ น้ำส้มสายชูยังใช้เพื่อกระตุ้นปฏิกิริยาเคมีที่เกิดขึ้นระหว่างการปรุงอาหารและการอบ ตัวอย่างเช่น เมื่อเบกกิ้งโซดาผสมกับกรด จะทำให้เกิดก๊าซติดอยู่ในแป้งหรือแป้งที่สร้างเนื้อสัมผัสที่นุ่มฟูเมื่อปรุงสุก น้ำส้มสายชูมักใช้ในน้ำหมักเพราะกรดช่วยให้เนื้อนุ่มโดยการทำลายเส้นใยของมัน ปกติแล้วน้ำส้มสายชูกลั่นสีขาวถูกใช้เป็นยาฆ่าเชื้อในบ้าน โดยเฉพาะอย่างยิ่งในห้องครัวและห้องน้ำ

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